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Crystal structure of beta'-COP/yWbp1 complex

X-ray diffraction
1.48Å resolution
PDB entry 4j86 coloured by chain, front view.
PDB entry 4j86 coloured by chain, side view.
PDB entry 4j86 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 and Coatomer subunit beta'
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Multimeric state: hetero dimer
Accessible surface area: 12007.52 Å2
Buried surface area: 718.4 Å2
Dissociation area: 359.2 Å2
Dissociation energy (ΔGdiss): -3.93 kcal/mol
Dissociation entropy (TΔSdiss): 6.05 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-152649
Assembly 2
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Assembly Name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 and Coatomer subunit beta'
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Multimeric state: hetero dimer
Accessible surface area: 12073.93 Å2
Buried surface area: 714.96 Å2
Dissociation area: 357.48 Å2
Dissociation energy (ΔGdiss): -4 kcal/mol
Dissociation entropy (TΔSdiss): 6.06 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-152649

Macromolecules

Coatomer subunit beta'
Chains: A, B
Length: 301 amino acids
Theoretical weight: 34.29 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P41811 (Residues: 1-301; Coverage: 34%)
Gene names: G2827, SEC27, YGL137W
Pfam: WD domain, G-beta repeat
InterPro:
CATH: YVTN repeat-like/Quinoprotein amine dehydrogenase
Coatomer subunit beta' in PDB entry 4j86, assembly 1, front view.
Coatomer subunit beta' in PDB entry 4j86, assembly 1, side view.
Coatomer subunit beta' in PDB entry 4j86, assembly 1, top view.
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Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1
Chains: C, D
Length: 6 amino acids
Theoretical weight: 740 Da
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P33767 (Residues: 425-430; Coverage: 2%)
Gene names: WBP1, YEL002C
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 in PDB entry 4j86, assembly 1, front view.
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 in PDB entry 4j86, assembly 1, side view.
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 in PDB entry 4j86, assembly 1, top view.
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