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Crystal structure of beta'-COP/Insig-1 complex

X-ray diffraction
1.745Å resolution
PDB entry 4j81 coloured by chain, front view.
PDB entry 4j81 coloured by chain, side view.
PDB entry 4j81 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Insulin-induced gene 1 protein and Coatomer subunit beta'
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Multimeric state: hetero dimer
Accessible surface area: 11757.44 Å2
Buried surface area: 766.75 Å2
Dissociation area: 383.38 Å2
Dissociation energy (ΔGdiss): 0.07 kcal/mol
Dissociation entropy (TΔSdiss): 5.68 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-127497
Assembly 2
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Assembly Name: Insulin-induced gene 1 protein and Coatomer subunit beta'
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Multimeric state: hetero dimer
Accessible surface area: 11831.93 Å2
Buried surface area: 769.66 Å2
Dissociation area: 384.83 Å2
Dissociation energy (ΔGdiss): 1.29 kcal/mol
Dissociation entropy (TΔSdiss): 5.67 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-127497

Macromolecules

Coatomer subunit beta'
Chains: A, B
Length: 301 amino acids
Theoretical weight: 34.29 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P41811 (Residues: 1-301; Coverage: 34%)
Gene names: G2827, SEC27, YGL137W
Pfam: WD domain, G-beta repeat
InterPro:
CATH: YVTN repeat-like/Quinoprotein amine dehydrogenase
Coatomer subunit beta' in PDB entry 4j81, assembly 1, front view.
Coatomer subunit beta' in PDB entry 4j81, assembly 1, side view.
Coatomer subunit beta' in PDB entry 4j81, assembly 1, top view.
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Insulin-induced gene 1 protein
Chains: C, D
Length: 5 amino acids
Theoretical weight: 585 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O15503 (Residues: 273-277; Coverage: 2%)
Gene name: INSIG1
Insulin-induced gene 1 protein in PDB entry 4j81, assembly 1, front view.
Insulin-induced gene 1 protein in PDB entry 4j81, assembly 1, side view.
Insulin-induced gene 1 protein in PDB entry 4j81, assembly 1, top view.
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