PDBe 4j4l

X-ray diffraction
2.3Å resolution

Modular evolution and design of the protein binding interface

Released:
Entry authors: Cheong HK, Kim HJ

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Interleukin-6 Chains: C, D
Molecule details ›
Chains: C, D
Length: 168 amino acids
Theoretical weight: 19.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05231 (Residues: 47-212; Coverage: 91%)
Gene names: IFNB2, IL6
Structure domains: Growth Hormone; Chain: A;
Internalin B; Variable lymphocyte receptor B Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 30.06 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: A4L9V2 (Residues: 43-122, 145-153; Coverage: 14%)
  • Canonical: Q4G1L3 (Residues: 54-77, 87-232; Coverage: 60%)
  • nullnull
Gene name: VLRB
Sequence domains: Leucine rich repeat
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P212121
Unit cell:
a: 46.319Å b: 134.001Å c: 148.722Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.268
Expression system: Escherichia coli