PDBe 4j3e

X-ray diffraction
1.91Å resolution

The 1.9A crystal structure of humanized Xenopus Mdm2 with nutlin-3a


Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
E3 ubiquitin-protein ligase Mdm2 Chain: A
Molecule details ›
Chain: A
Length: 86 amino acids
Theoretical weight: 9.96 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21
  • Canonical: P56273 (Residues: 20-105; Coverage: 18%)
Gene name: mdm2
Sequence domains: SWIB/MDM2 domain
Structure domains: MDM2

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: C2221
Unit cell:
a: 42.887Å b: 67.657Å c: 67.173Å
α: 90° β: 90° γ: 90°
R R work R free
0.196 0.194 0.239
Expression system: Escherichia coli BL21