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Crystal structure of ZNF217 bound to DNA

X-ray diffraction
2.1Å resolution
PDB entry 4is1 coloured by chain, front view.
PDB entry 4is1 coloured by chain, side view.
PDB entry 4is1 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Zinc finger protein 217 and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 12937.62 Å2
Buried surface area: 5615.71 Å2
Dissociation area: 843.71 Å2
Dissociation energy (ΔGdiss): 22.77 kcal/mol
Dissociation entropy (TΔSdiss): 10.81 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-117660

Macromolecules

Zinc finger protein 217
Chains: C, D
Length: 57 amino acids
Theoretical weight: 6.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75362 (Residues: 469-523; Coverage: 5%)
Gene names: ZABC1, ZNF217
Pfam: Zinc finger, C2H2 type
InterPro:
CATH: Classic Zinc Finger
Zinc finger protein 217 in PDB entry 4is1, assembly 1, front view.
Zinc finger protein 217 in PDB entry 4is1, assembly 1, side view.
Zinc finger protein 217 in PDB entry 4is1, assembly 1, top view.
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DNA chain A
Name: 5'-D(*TP*TP*TP*GP*CP*AP*GP*AP*AP*TP*CP*GP*AP*TP*TP*CP*TP*GP*CP*A)-3'
Representative chains: A
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 20 nucleotides
Theoretical weight: 6.12 KDa
5'-D(*TP*TP*TP*GP*CP*AP*GP*AP*AP*TP*CP*GP*AP*TP*TP*CP*TP*GP*CP*A)-3' in PDB entry 4is1, assembly 1, front view.
5'-D(*TP*TP*TP*GP*CP*AP*GP*AP*AP*TP*CP*GP*AP*TP*TP*CP*TP*GP*CP*A)-3' in PDB entry 4is1, assembly 1, side view.
5'-D(*TP*TP*TP*GP*CP*AP*GP*AP*AP*TP*CP*GP*AP*TP*TP*CP*TP*GP*CP*A)-3' in PDB entry 4is1, assembly 1, top view.
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DNA chain B
Name: 5'-D(*AP*AP*TP*GP*CP*AP*GP*AP*AP*TP*CP*GP*AP*TP*TP*CP*TP*GP*CP*A)-3'
Representative chains: B
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 20 nucleotides
Theoretical weight: 6.14 KDa
5'-D(*AP*AP*TP*GP*CP*AP*GP*AP*AP*TP*CP*GP*AP*TP*TP*CP*TP*GP*CP*A)-3' in PDB entry 4is1, assembly 1, front view.
5'-D(*AP*AP*TP*GP*CP*AP*GP*AP*AP*TP*CP*GP*AP*TP*TP*CP*TP*GP*CP*A)-3' in PDB entry 4is1, assembly 1, side view.
5'-D(*AP*AP*TP*GP*CP*AP*GP*AP*AP*TP*CP*GP*AP*TP*TP*CP*TP*GP*CP*A)-3' in PDB entry 4is1, assembly 1, top view.
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