› 4iri

X-ray diffraction
2.77Å resolution

Auto-inhibited ERG Ets Domain-DNA Complex

Released:
DOI: 10.2210/pdb4iri/pdb
PDB entry 4iri coloured by chain, front view.
PDB entry 4iri coloured by chain, side view.
PDB entry 4iri coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and dynamic studies of the transcription factor ERG reveal DNA binding is allosterically autoinhibited.
Regan MC, Horanyi PS, Pryor EE, Sarver JL, Cafiso DS, Bushweller JH
Proc Natl Acad Sci U S A 110 13374-9 (2013)
PMID: 23898196

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117658 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional regulator ERG Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11308 (Residues: 280-405; Coverage: 26%)
Gene name: ERG
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*GP*AP*CP*CP*GP*GP*AP*AP*GP*TP*GP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.75 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*CP*AP*CP*TP*TP*CP*CP*GP*GP*TP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.57 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P4122
Unit cell:
a: 51.486Å b: 51.486Å c: 141.313Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.21
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

The oncogene ERG: a key factor in prostate cancer.
Adamo et al. (2016)
4 more

13 mentions without citation

ERF mutations reveal a balance of ETS factors controlling prostate oncogenesis.
Bose et al. (2017)
12 more