› 4iqr

X-ray diffraction
2.9Å resolution

Multi-Domain Organization of the HNF4alpha Nuclear Receptor Complex on DNA

Released:
DOI: 10.2210/pdb4iqr/pdb
PDB entry 4iqr coloured by chain, front view.
PDB entry 4iqr coloured by chain, side view.
PDB entry 4iqr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Multidomain integration in the structure of the HNF-4α nuclear receptor complex.
Chandra V, Huang P, Potluri N, Wu D, Kim Y, Rastinejad F
Nature 495 394-8 (2013)
PMID: 23485969

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117651 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Hepatocyte nuclear factor 4-alpha Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 338 amino acids
Theoretical weight: 38.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P41235 (Residues: 55-377; Coverage: 68%)
Gene names: HNF4, HNF4A, NR2A1, TCF14
Sequence domains:
Structure domains:
Nuclear receptor coactivator 2 Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 13 amino acids
Theoretical weight: 1.62 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 685-697; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2
DNA (5'-D(*GP*GP*AP*AP*CP*TP*AP*GP*GP*TP*CP*AP*AP*AP*GP*GP*TP*CP*AP*G)-3') Chains: C, G
Molecule details ›
Chains: C, G
Length: 20 nucleotides
Theoretical weight: 6.23 KDa
DNA (5'-D(*CP*CP*TP*GP*AP*CP*CP*TP*TP*TP*GP*AP*CP*CP*TP*AP*GP*TP*TP*C)-3') Chains: D, H
Molecule details ›
Chains: D, H
Length: 20 nucleotides
Theoretical weight: 6.04 KDa

Ligands and Environments

2 bound ligands:
Ligand MYR 4 x MYR
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 119.786Å b: 57.596Å c: 166.305Å
α: 90° β: 96.3° γ: 90°
R-values:
R R work R free
0.241 0.239 0.278
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

28 review citations

The nuclear receptor superfamily: A structural perspective.
Weikum et al. (2018)
27 more

25 mentions without citation

Androgen receptor: structure, role in prostate cancer and drug discovery.
Tan et al. (2015)
24 more