PDBe 4imy

X-ray diffraction
2.94Å resolution

The AFF4 scaffold binds human P-TEFb adjacent to HIV Tat

Released:

Function and Biology Details

Reactions catalysed:
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cyclin-dependent kinase 9 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 332 amino acids
Theoretical weight: 38.23 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P50750 (Residues: 1-330; Coverage: 89%)
Gene names: CDC2L4, CDK9, TAK
Sequence domains: Protein kinase domain
Structure domains:
Cyclin-T1 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 264 amino acids
Theoretical weight: 30.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O60563 (Residues: 1-264; Coverage: 36%)
Gene name: CCNT1
Sequence domains: Cyclin, N-terminal domain
Structure domains: Cyclin-like
AF4/FMR2 family member 4 Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 75 amino acids
Theoretical weight: 8.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHB7 (Residues: 2-73; Coverage: 6%)
Gene names: AF5Q31, AFF4, HSPC092, MCEF

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 100.691Å b: 126.298Å c: 195.626Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.245
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli