PDBe 4imx

X-ray diffraction
2.25Å resolution

Structure of bovine endothelial nitric oxide synthase heme domain in complex with 3,5-bis(2-(6-amino-4-methylpyridin-2-yl)ethyl)benzonitrile

Released:

Function and Biology Details

Reaction catalysed:
2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase, endothelial Chains: A, B
Molecule details ›
Chains: A, B
Length: 442 amino acids
Theoretical weight: 49.48 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29473 (Residues: 40-482; Coverage: 37%)
Gene name: NOS3
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 58.057Å b: 106.604Å c: 156.464Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.222
Expression system: Escherichia coli BL21(DE3)