› 4ilz

X-ray diffraction
1.91Å resolution

Structural and kinetic study of an internal substrate binding site of dehaloperoxidase-hemoglobin A from Amphitrite ornata

Released:
DOI: 10.2210/pdb4ilz/pdb
PDB entry 4ilz coloured by chain, front view.
PDB entry 4ilz coloured by chain, side view.
PDB entry 4ilz coloured by chain, top view.
Source organism: Amphitrite ornata
Primary publication:
Structural and kinetic study of an internal substrate binding site in dehaloperoxidase-hemoglobin A from Amphitrite ornata.
Zhao J, de Serrano V, Zhao J, Le P, Franzen S
Biochemistry 52 2427-39 (2013)
PMID: 23480178

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Globin domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 137 amino acids
Theoretical weight: 15.55 KDa
Source organism: Amphitrite ornata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NAV8 (Residues: 2-138; Coverage: 99%)
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

5 bound ligands:
Ligand HEM 2 x HEM
Ligand SO4 4 x SO4
Ligand IPA 7 x IPA
Ligand TBP 1 x TBP
Ligand OXY 1 x OXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 57.589Å b: 67.184Å c: 68.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.24 0.285
Expression system: Escherichia coli

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Citations

1 review citation

Carbon-fluorine bond cleavage mediated by metalloenzymes.
Wang et al. (2020)
7 other articles