4ijm

X-ray diffraction
3.35Å resolution

Crystal structure of circadian clock protein KaiC A422V mutant

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
PDBe Complex ID:
PDB-CPX-181278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Circadian clock oscillator protein KaiC Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 512 amino acids
Theoretical weight: 57.64 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79PF4 (Residues: 14-519; Coverage: 98%)
Gene names: Synpcc7942_1216, kaiC, see0011
Sequence domains: KaiC
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 131.362Å b: 134.468Å c: 203.936Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.261
Expression system: Escherichia coli BL21(DE3)