› 4iew

X-ray diffraction
1.45Å resolution

Cys-only bound Cysteine Dioxygenase at pH 9.0 in the presence of Cys

Released:
DOI: 10.2210/pdb4iew/pdb
PDB entry 4iew coloured by chain, front view.
PDB entry 4iew coloured by chain, side view.
PDB entry 4iew coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Cysteine dioxygenase structures from pH4 to 9: consistent cys-persulfenate formation at intermediate pH and a Cys-bound enzyme at higher pH.
Driggers CM, Cooley RB, Sankaran B, Hirschberger LL, Stipanuk MH, Karplus PA
J Mol Biol 425 3121-36 (2013)
PMID: 23747973

Function and Biology Details

Reaction catalysed: 
L-cysteine + O(2) = 3-sulfinoalanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine dioxygenase type 1 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 23.06 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P21816 (Residues: 1-200; Coverage: 100%)
Gene name: Cdo1
Sequence domains: Cysteine dioxygenase type I
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand FE2 1 x FE2
Ligand CYS 1 x CYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P43212
Unit cell:
a: 57.6Å b: 57.6Å c: 122.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.149 0.199
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Chemical Biology of H2S Signaling through Persulfidation.
Filipovic et al. (2018)
3 more

1 mention without citation

Cysteine dioxygenase structures from pH4 to 9: consistent cys-persulfenate formation at intermediate pH and a Cys-bound enzyme at higher pH.
Driggers et al. (2013)