PDBe 4i9q

X-ray diffraction
2.3Å resolution

Crystal structure of the ternary complex of the D714A mutant of RB69 DNA polymerase

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-directed DNA polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 903 amino acids
Theoretical weight: 104.52 KDa
Source organism: Enterobacteria phage RB69
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38087 (Residues: 1-903; Coverage: 100%)
Gene name: 43
Sequence domains:
Structure domains:
DNA (5'-D(*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*C)-3') Chains: C, P
Molecule details ›
Chains: C, P
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*CP*AP*CP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G)-3') Chains: D, T
Molecule details ›
Chains: D, T
Length: 18 nucleotides
Theoretical weight: 5.5 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 73.781Å b: 119.412Å c: 146.016Å
α: 90° β: 90.26° γ: 90°
R-values:
R R work R free
0.254 0.252 0.287
Expression systems:
  • Escherichia coli
  • Not provided