› 4hun

X-ray diffraction
3.59Å resolution

MATE transporter NorM-NG in complex with R6G and monobody

Released:
DOI: 10.2210/pdb4hun/pdb
PDB entry 4hun coloured by chain, front view.
PDB entry 4hun coloured by chain, side view.
PDB entry 4hun coloured by chain, top view.
Source organisms:
Primary publication:
Structures of a Na+-coupled, substrate-bound MATE multidrug transporter.
Lu M, Symersky J, Radchenko M, Koide A, Guo Y, Nie R, Koide S
Proc Natl Acad Sci U S A 110 2099-104 (2013)
PMID: 23341609

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-125914 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Multidrug efflux protein Chain: A
Molecule details ›
Chain: A
Length: 459 amino acids
Theoretical weight: 49.72 KDa
Source organism: Neisseria gonorrhoeae TCDC-NG08107
Expression system: Escherichia coli
Fibronectin type-III domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 10.92 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: M1E1G6 (Residues: 1-99; Coverage: 100%)
Sequence domains: Fibronectin type III domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand RHQ 1 x RHQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P3221
Unit cell:
a: 117.234Å b: 117.234Å c: 226.218Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.307 0.306 0.327
Expression system: Escherichia coli

Quick links

Citations

22 review citations

The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.
Li et al. (2015)
21 more

6 mentions without citation

Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases.
Kim et al. (2021)
5 more