PDBe 4htp

X-ray diffraction
2.25Å resolution

Crystal structure of the DBD domain of human DNA ligase IV bound to Artemis peptide

Released:

Function and Biology Details

Reaction catalysed:
ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho- (deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA ligase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 240 amino acids
Theoretical weight: 27.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49917 (Residues: 1-240; Coverage: 26%)
Gene name: LIG4
Sequence domains: DNA ligase N terminus
Protein artemis Chains: C, E
Molecule details ›
Chains: C, E
Length: 11 amino acids
Theoretical weight: 1.45 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96SD1 (Residues: 485-495; Coverage: 2%)
Gene names: ARTEMIS, ASCID, DCLRE1C, SCIDA, SNM1C

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 61.21Å b: 72.83Å c: 158.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.249
Expression systems:
  • Escherichia coli
  • Not provided