› 4hr5

X-ray diffraction
2.29Å resolution

R2-like ligand-binding oxidase without metal cofactor

Released:
DOI: 10.2210/pdb4hr5/pdb
PDB entry 4hr5 coloured by chain, front view.
PDB entry 4hr5 coloured by chain, side view.
PDB entry 4hr5 coloured by chain, top view.
Source organism: Geobacillus kaustophilus HTA426
Primary publication:
Direct observation of structurally encoded metal discrimination and ether bond formation in a heterodinuclear metalloprotein.
Griese JJ, Roos K, Cox N, Shafaat HS, Branca RM, Lehtiö J, Gräslund A, Lubitz W, Siegbahn PE, Högbom M
Proc Natl Acad Sci U S A 110 17189-94 (2013)
PMID: 24101498

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177364 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
R2-like ligand binding oxidase Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 36.96 KDa
Source organism: Geobacillus kaustophilus HTA426
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5KW80 (Residues: 1-302; Coverage: 100%)
Gene name: GK2771
Sequence domains: Ribonucleotide reductase, small chain
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

1 bound ligand:
Ligand PLM 1 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: I222
Unit cell:
a: 56.757Å b: 97.043Å c: 127.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.237
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Dioxygen Activation by Nonheme Diiron Enzymes: Diverse Dioxygen Adducts, High-Valent Intermediates, and Related Model Complexes.
Jasniewski et al. (2018)
3 more

1 mention without citation

Direct observation of structurally encoded metal discrimination and ether bond formation in a heterodinuclear metalloprotein.
Griese et al. (2013)