› 4hpu

X-ray diffraction
1.55Å resolution

Crystal structure of the catalytic subunit of cAMP-dependent protein kinase displaying partial phosphoryl transfer of AMP-PNP onto a substrate peptide

Released:
DOI: 10.2210/pdb4hpu/pdb
PDB entry 4hpu coloured by chain, front view.
PDB entry 4hpu coloured by chain, side view.
PDB entry 4hpu coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Phosphoryl transfer by protein kinase A is captured in a crystal lattice.
Bastidas AC, Deal MS, Steichen JM, Guo Y, Wu J, Taylor SS
J Am Chem Soc 135 4788-98 (2013)
PMID: 23458248

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138546 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 350 amino acids
Theoretical weight: 40.74 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase inhibitor alpha Chain: I
Molecule details ›
Chain: I
Length: 20 amino acids
Theoretical weight: 2.28 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P63248 (Residues: 6-25; Coverage: 26%)
Gene name: Pkia

Ligands and Environments

3 bound ligands:
Ligand MYR 1 x MYR
Ligand ANP 2 x ANP
Ligand MG 2 x MG
2 modified residues:
Ligand SEP 3 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 57.69Å b: 79.8Å c: 98.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.194
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Src protein-tyrosine kinase structure, mechanism, and small molecule inhibitors.
Roskoski R. (2015)
6 more

5 mentions without citation

Molecular basis for small molecule inhibition of G protein-coupled receptor kinases.
Homan et al. (2015)
4 more