› 4hp5

X-ray diffraction
2Å resolution

The crystal structure of isomaltulose synthase mutant E295A from Erwinia rhapontici NX5 in complex with D-glucose

Released:
DOI: 10.2210/pdb4hp5/pdb
PDB entry 4hp5 coloured by chain, front view.
PDB entry 4hp5 coloured by chain, side view.
PDB entry 4hp5 coloured by chain, top view.
Source organism: Erwinia rhapontici
Entry authors: Xu Z, Li S, Xu H, Zhou J

Function and Biology Details

Reaction catalysed: 
Sucrose = 6-O-alpha-D-glucopyranosyl-D-fructofuranose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112985 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 559 amino acids
Theoretical weight: 65.77 KDa
Source organism: Erwinia rhapontici
Expression system: Escherichia coli
UniProt:
  • Canonical: D9MPF2 (Residues: 42-600; Coverage: 93%)
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand CA 1 x CA
Ligand GLC 1 x GLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 58.849Å b: 81.325Å c: 138.258Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.154 0.208
Expression system: Escherichia coli

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