PDBe 4hoe

X-ray diffraction
1.76Å resolution

Candida albicans dihydrofolate reductase complexed with NADPH and 5-[3-(2,5-dimethoxy-4-phenylphenyl)but-1-yn-1-yl]-6-methylpyrimidine-2,4-diamine (UCP111E)


Function and Biology Details

Reaction catalysed:
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Dihydrofolate reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 22.19 KDa
Source organism: Candida albicans
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P22906 (Residues: 1-192; Coverage: 100%)
Gene name: DFR1
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 38.003Å b: 66.538Å c: 75.795Å
α: 90° β: 93.11° γ: 90°
R R work R free
0.216 0.182 0.216
Expression system: Escherichia coli BL21(DE3)