PDBe 4hlu

X-ray diffraction
2.7Å resolution

Structure of the EcfA-A' heterodimer bound to ADP

Released:
Source organism: Thermotoga maritima MSB8
Primary publication:
Assembly and mechanism of a group II ECF transporter.
Proc. Natl. Acad. Sci. U.S.A. 110 2534-9 (2013)
PMID: 23359690

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Energy-coupling factor transporter ATP-binding protein EcfA2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 268 amino acids
Theoretical weight: 30.39 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WY65 (Residues: 2-266; Coverage: 100%)
Gene names: TM_0222, ecfA, ecfA', ecfA2
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Energy-coupling factor transporter ATP-binding protein EcfA1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 268 amino acids
Theoretical weight: 29.86 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X1Z1 (Residues: 2-259; Coverage: 100%)
Gene names: TM_1663, cbiO, ecfA', ecfA1
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P32
Unit cell:
a: 67.785Å b: 67.785Å c: 252.509Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.213 0.258
Expression system: Escherichia coli BL21(DE3)