PDBe 4hkt

X-ray diffraction
2Å resolution

Crystal structure of a putative myo-inositol dehydrogenase from Sinorhizobium meliloti 1021 (Target PSI-012312)

Released:
Source organism: Sinorhizobium meliloti 1021
Entry authors: Sampathkumar P, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed:
Myo-inositol + NAD(+) = 2,4,6/3,5-pentahydroxycyclohexanone + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol 2-dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 331 amino acids
Theoretical weight: 35.61 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O68965 (Residues: 1-330; Coverage: 100%)
Gene names: RB1194, SMb20899, idhA, iolG
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 90.449Å b: 55.467Å c: 149.091Å
α: 90° β: 97.56° γ: 90°
R-values:
R R work R free
0.188 0.186 0.232
Expression system: Escherichia coli BL21(DE3)