› 4hi0

X-ray diffraction
2.35Å resolution

Crystal Structure of Helicobacter pylori Urease Accessory Protein UreF/H/G complex

Released:
DOI: 10.2210/pdb4hi0/pdb
PDB entry 4hi0 coloured by chain, front view.
PDB entry 4hi0 coloured by chain, side view.
PDB entry 4hi0 coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
Structure of UreG/UreF/UreH complex reveals how urease accessory proteins facilitate maturation of Helicobacter pylori urease.
Fong YH, Wong HC, Yuen MH, Lau PH, Chen YW, Wong KB
PLoS Biol 11 e1001678 (2013)
PMID: 24115911

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-170761 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Urease accessory protein UreF Chains: A, C
Molecule details ›
Chains: A, C
Length: 254 amino acids
Theoretical weight: 28.65 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09065 (Residues: 1-254; Coverage: 100%)
Gene names: HP_0069, ureF
Sequence domains: UreF
Structure domains: Urease accessory protein UreF
Urease accessory protein UreH Chains: B, D
Molecule details ›
Chains: B, D
Length: 265 amino acids
Theoretical weight: 29.76 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09067 (Residues: 1-265; Coverage: 100%)
Gene names: HP_0067, ureH
Sequence domains: UreD urease accessory protein
Urease accessory protein UreG Chains: E, F
Molecule details ›
Chains: E, F
Length: 199 amino acids
Theoretical weight: 21.98 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09066 (Residues: 1-199; Coverage: 100%)
Gene names: HP_0068, ureG
Sequence domains: CobW/HypB/UreG, nucleotide-binding domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 76.124Å b: 96.429Å c: 237.452Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.242
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Common themes and unique proteins for the uptake and trafficking of nickel, a metal essential for the virulence of Helicobacter pylori.
de Reuse et al. (2013)
7 more

21 mentions without citation

Metallochaperones and metalloregulation in bacteria.
Capdevila et al. (2017)
20 more