› 4hhv

X-ray diffraction
1.75Å resolution

Crystal structure of ceramide transfer protein pleckstrin homology domain

Released:
DOI: 10.2210/pdb4hhv/pdb
PDB entry 4hhv coloured by chain, front view.
PDB entry 4hhv coloured by chain, side view.
PDB entry 4hhv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the pleckstrin homology domain from the ceramide transfer protein: implications for conformational change upon ligand binding.
Prashek J, Truong T, Yao X
PLoS One 8 e79590 (2013)
PMID: 24260258

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195282 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ceramide transfer protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 105 amino acids
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y5P4 (Residues: 20-121; Coverage: 16%)
Gene names: CERT, CERT1, COL4A3BP, STARD11
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

4 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 3 x GOL
Ligand CL 3 x CL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 48.131Å b: 54.979Å c: 98.988Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.204
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The Oxysterol-Binding Protein Cycle: Burning Off PI(4)P to Transport Cholesterol.
Antonny et al. (2018)
5 more

4 mentions without citation

Interaction between the PH and START domains of ceramide transfer protein competes with phosphatidylinositol 4-phosphate binding by the PH domain.
Prashek et al. (2017)
3 more