4hf1

X-ray diffraction
2.22Å resolution

Crystal Structure of IscR bound to its promoter

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-117585 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH-type transcriptional regulator IscR Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 18.21 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGK8 (Residues: 1-162; Coverage: 100%)
Gene names: JW2515, b2531, iscR, yfhP
Sequence domains: Iron-dependent Transcriptional regulator
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (29-MER) Chain: C
Molecule details ›
Chain: C
Length: 29 nucleotides
Theoretical weight: 8.89 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (29-MER) Chain: D
Molecule details ›
Chain: D
Length: 29 nucleotides
Theoretical weight: 8.93 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 49.124Å b: 75.32Å c: 187.394Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.228
Expression systems:
  • Escherichia coli
  • Not provided