PDBe 4hel

X-ray diffraction
3.2Å resolution

Crystal structure analysis of apo-GroEL structure

Released:
Source organism: Escherichia coli
Entry authors: Saxena AK, Meena SR

Function and Biology Details

Structure analysis Details

Assembly composition:
homo 14-mer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
60 kDa chaperonin Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 525 amino acids
Theoretical weight: 55.35 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: Q548M1 (Residues: 2-526; Coverage: 96%)
Gene names: A8C65_01580, A8G17_01625, A8M42_07850, A9R57_13545, AA102_13095, AC067_05505, AC789_1c45570, ACN002_4372, ACN77_13925, ACN81_06325, ACU57_19295, ACU90_27610, AJ318_17205, AKG99_18195, AM266_00725, AM270_14500, AM464_15770, AMK83_27450, AML35_20705, APT94_02245, APZ14_01135, ARC77_17990, AU473_28145, AUQ13_10300, AUS26_18735, AW059_20455, AW106_26290, AZE01_18030, B1K96_03090, B7C53_14115, B9T59_10250, BB545_11040, BE963_12230, BEN53_00765, BER14_11410, BH694_19650, BHF46_15625, BHS81_24830, BHS87_23225, BIQ87_23895, BIU72_06095, BIZ41_02710, BJJ90_23450, BK248_23630, BK292_23445, BK334_08295, BK373_03440, BK375_06855, BK383_12940, BK400_02730, BMT49_19180, BMT53_11945, BMT91_05495, BN17_41231, BSR05_14030, BTQ04_18135, BTQ06_07135, BUE81_24315, BVL39_04415, BW690_22405, BWP17_13605, BXT93_23590, BZL31_12950, BZL69_06910, C1I39_24100, C1I57_02515, C2858_12005, C2M16_07145, C2U48_11800, C3K24_17540, C4K41_15740, C4M78_05800, C5715_24370, C5N07_07010, C5P01_11485, C5P43_04990, C5P44_18550, C5Y95_14435, C6976_23915, C7235_22800, C7B02_22870, C7B06_19620, C7B07_19640, C7B08_13320, C9E25_08280, CA593_05105, CCZ05_11870, CCZ08_19865, CCZ11_19940, CCZ14_19960, CCZ16_17425, CCZ17_16290, CCZ18_09570, CCZ21_16815, CDL37_19955, CIJ94_19305, COD30_28660, COD46_17610, CR539_02660, CRM83_17505, CRT55_26565, CSB64_20580, CT143_14485, CT146_11195, CU078_13310, CUB99_10685, CV83915_01888, CVH05_06685, CWM24_06435, CWS33_03390, CXB56_25445, DD647_07895, DD762_21030, DDT63_05215, DIV10_26330, DL800_29220, DM463_13480, DNK79_13900, DNQ41_02325, DNQ45_22695, DNR41_18215, DPC53_01315, DQE83_13435, EC1094V2_4111, EC3234A_108c00480, EC95NR1_03760, ECONIH1_24685, ECs5124, EL75_4022, EL79_4200, EL80_4116, ERS085365_01231, ERS085366_02367, ERS085374_01721, ERS085379_04164, ERS085383_01552, ERS085386_01025, ERS085404_01348, ERS085416_03698, ERS139211_04024, ERS150873_01289, ERS150876_03930, FORC28_5528, GJ11_26030, HMPREF3040_03032, JD73_27620, MJ49_08265, MS6198_48630, MS8345_04771, NCTC10650_05230, NCTC11023_04163, NCTC11123_00565, NCTC11560_04827, NCTC13353_01582, NCTC13462_02684, NCTC8007_03304, NCTC9073_03984, NCTC9111_04720, NCTC9777_00950, PGD_03325, PU06_23700, RG28_06025, RK56_012245, RX35_04405, SAMEA3472031_02428, SAMEA3472033_01789, SAMEA3472035_05052, SAMEA3472039_02867, SAMEA3472046_00148, SAMEA3472049_03491, SAMEA3472052_01140, SAMEA3472053_01212, SAMEA3472055_04142, SAMEA3472056_01662, SAMEA3472064_04073, SAMEA3472067_02182, SAMEA3472078_00113, SAMEA3472079_04965, SAMEA3472089_02866, SAMEA3472090_01020, SAMEA3472113_01085, SAMEA3472114_01908, SAMEA3472115_00492, SAMEA3472117_05492, SAMEA3472119_01136, SAMEA3472121_05492, SAMEA3472127_01593, SAMEA3472140_02903, SAMEA3472152_03191, SAMEA3484419_01874, SAMEA3484423_00439, SAMEA3484427_02051, SAMEA3484429_03091, SAMEA3484434_04204, SAMEA3484438_04735, SAMEA3484441_01161, SAMEA3484442_04618, SAMEA3484444_03154, SAMEA3484446_03267, SAMEA3484447_01145, SAMEA3485083_01541, SAMEA3485084_01087, SAMEA3485088_00777, SAMEA3485090_02055, SAMEA3485095_01880, SAMEA3485098_03608, SAMEA3485101_04769, SAMEA3485104_01038, SAMEA3485106_00442, SAMEA3485110_03208, SAMEA3485111_04197, SAMEA3485112_04308, SAMEA3485113_03474, SAMEA3485115_01402, SAMEA3751239_02348, SAMEA3751242_03600, SAMEA3751243_01398, SAMEA3751392_02800, SAMEA3751400_04220, SAMEA3751407_02300, SAMEA3752267_02924, SAMEA3752270_02478, SAMEA3752310_01825, SAMEA3752311_01061, SAMEA3752316_01105, SAMEA3752372_01184, SAMEA3752378_01902, SAMEA3752379_00847, SAMEA3752509_03038, SAMEA3752555_02224, SAMEA3752556_04264, SAMEA3752558_01564, SAMEA3752560_00597, SAMEA3752562_01862, SAMEA3752612_01018, SAMEA3752618_03868, SAMEA3752619_02567, SAMEA3752685_02917, SAMEA3752686_03215, SAMEA3752694_03145, SAMEA3752739_02419, SAMEA3753064_01580, SAMEA3753068_01675, SAMEA3753069_01294, SAMEA3753070_03223, SAMEA3753072_01309, SAMEA3753074_02873, SAMEA3753075_03138, SAMEA3753077_02956, SAMEA3753091_03405, SAMEA3753092_00963, SAMEA3753094_04106, SAMEA3753097_01210, SAMEA3753098_03124, SAMEA3753101_01954, SAMEA3753105_02626, SAMEA3753106_03412, SAMEA3753137_03297, SAMEA3753141_02077, SAMEA3753157_02414, SAMEA3753158_03362, SAMEA3753159_01010, SAMEA3753160_03474, SAMEA3753161_03615, SAMEA3753290_02069, SAMEA3753294_00181, SAMEA3753300_00682, SAMEA3753303_02512, SAMEA3753304_04545, SAMEA3753305_03058, SAMEA3753307_02333, SAMEA3753355_03063, SAMEA3753391_00661, SAMEA3753396_03226, SAMEA3753397_02631, SAMEA3753398_00792, SAMEA3753401_03647, SAMEA3753404_03816, SK85_04506, SY51_23895, UC41_03555, UN91_24955, WQ89_21775, WR15_07175, YDC107_2866, groEL, groL, mopA
Sequence domains: TCP-1/cpn60 chaperonin family
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 136.24Å b: 261.83Å c: 282.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.223