PDBe 4hel

X-ray diffraction
3.2Å resolution

Crystal structure analysis of apo-GroEL structure

Released:
Source organism: Escherichia coli
Entry authors: Saxena AK, Meena SR

Function and Biology Details

Structure analysis Details

Assembly composition:
homo 14-mer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
60 kDa chaperonin Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 525 amino acids
Theoretical weight: 55.35 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: Q548M1 (Residues: 2-526; Coverage: 96%)
Gene names: A6581_03125, A8C65_01580, A8G17_01625, A8M42_07850, A9R57_13545, AC067_05505, AC789_1c45570, ACN002_4372, ACN77_13925, ACN81_06325, ACU57_19295, ACU90_27610, AKG99_18195, AM270_14500, AM446_25370, AM464_15770, AM465_02150, AMK83_27450, AML35_20705, APT13_02995, APT94_02245, APZ14_01135, ARC77_17990, AU473_28145, AUQ13_10300, AUS26_18735, AW059_20455, AW106_26290, B1K96_03090, B7C53_14115, B9M99_07795, B9N33_15905, B9T59_10250, BB545_11040, BE963_12230, BEN53_00765, BER14_11410, BH694_19650, BHF46_15625, BHS81_24830, BHS87_23225, BIQ87_23895, BIU72_06095, BIZ41_02710, BJJ90_23450, BK248_23630, BK292_23445, BK334_08295, BK373_03440, BK375_06855, BK383_12940, BK400_02730, BMT49_19180, BMT53_11945, BMT91_05495, BN17_41231, BSR05_14030, BTQ04_18135, BTQ06_07135, BUE81_24315, BVL39_04415, BW690_22405, BWP17_13605, BXT93_23590, BZL31_12950, BZL69_06910, C1I57_02515, C2M16_07145, C2U48_11800, C3K24_17540, C4K41_15740, C4M78_05800, C5715_24370, C5N07_07010, C5P01_11485, C5P43_04990, C5P44_18550, C6669_06700, C6B13_11455, C7235_22800, C7B02_22870, C7B06_19620, C7B07_19640, C7B08_13320, C9E25_08280, CA593_05105, CA902_11950, CCZ14_19960, CCZ17_16290, CDL37_19955, CG692_00055, CIJ94_19305, COD30_28660, COD46_17610, CRD98_26740, CRE02_12605, CRE03_25435, CRE06_15175, CRM83_17505, CSB64_20580, CT143_14485, CT146_11195, CUB99_10685, CV83915_01888, CVH05_06685, CWM24_06435, CWS33_03390, CXB56_25445, D0X26_12780, DD762_21030, DIV10_26330, DL545_22730, DL800_29220, DM463_13480, DNQ41_02325, DNQ45_22695, DNR41_18215, DQE83_13435, DS732_02495, DS966_08020, DTL41_15645, DTL43_02185, DTL84_03470, DTL90_17410, DTM10_04525, DTM25_10765, DTM27_17440, DTM45_21115, DVJ82_002280, EC1094V2_4111, EC3234A_108c00480, EC95NR1_03760, ECONIH1_24685, ECs5124, EL75_4022, EL79_4200, EL80_4116, ERS085365_01231, ERS085366_02367, ERS085374_01721, ERS085379_04164, ERS085383_01552, ERS085386_01025, ERS085404_01348, ERS085416_03698, ERS139211_04024, ERS150873_01289, ERS150876_03930, FORC28_5528, GJ11_26030, HMPREF3040_03032, JD73_27620, MJ49_08265, MS6198_48630, MS8345_04771, NCTC10082_01857, NCTC10090_02607, NCTC10418_06721, NCTC10764_04513, NCTC10767_00467, NCTC10865_05650, NCTC11022_04473, NCTC11112_02719, NCTC11181_01789, NCTC11341_03019, NCTC11560_04827, NCTC12950_04949, NCTC13125_02650, NCTC13127_05944, NCTC13462_02684, NCTC13846_04307, NCTC13919_01679, NCTC7152_04524, NCTC7927_05027, NCTC8450_00576, NCTC8500_05095, NCTC8621_04680, NCTC8622_02021, NCTC8959_04316, NCTC8960_02051, NCTC9007_00921, NCTC9010_04689, NCTC9036_04433, NCTC9037_04611, NCTC9045_05354, NCTC9050_02551, NCTC9055_01424, NCTC9058_02377, NCTC9062_03620, NCTC9073_03984, NCTC9077_05690, NCTC9081_01494, NCTC9110_04320, NCTC9111_04720, NCTC9117_05641, NCTC9119_04735, NCTC9434_02173, NCTC9701_04822, NCTC9703_03911, NCTC9706_01822, NCTC9775_02929, NCTC9777_00950, NCTC9969_04590, PU06_23700, RG28_06025, RK56_012245, RX35_04405, SAMEA3472033_01789, SAMEA3472044_01554, SAMEA3472047_01720, SAMEA3472055_04142, SAMEA3472056_01662, SAMEA3472067_02182, SAMEA3472070_02799, SAMEA3472080_00128, SAMEA3472089_02866, SAMEA3472090_01020, SAMEA3472110_00754, SAMEA3472112_01087, SAMEA3472114_01908, SAMEA3484427_02051, SAMEA3484429_03091, SAMEA3484433_02853, SAMEA3484434_04204, SAMEA3485101_04769, SAMEA3485103_02882, SAMEA3485113_03474, SAMEA3752372_01184, SAMEA3752379_00847, SAMEA3752559_00182, SAMEA3752620_03454, SAMEA3753064_01580, SAMEA3753097_01210, SAMEA3753106_03412, SAMEA3753164_04038, SAMEA3753290_02069, SAMEA3753300_00682, SAMEA3753391_00661, SAMEA3753397_02631, SK85_04506, SY51_23895, UC41_03555, UN91_24955, WQ89_21775, WR15_07175, YDC107_2866, groEL, groL, mopA
Sequence domains: TCP-1/cpn60 chaperonin family
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 136.24Å b: 261.83Å c: 282.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.223