› 4h8i

X-ray diffraction
2Å resolution

Structure of GluK2-LBD in complex with GluAzo

Released:
DOI: 10.2210/pdb4h8i/pdb
PDB entry 4h8i coloured by chain, front view.
PDB entry 4h8i coloured by chain, side view.
PDB entry 4h8i coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
A photoswitchable neurotransmitter analogue bound to its receptor.
Reiter A, Skerra A, Trauner D, Schiefner A
Biochemistry 52 8972-4 (2013)
PMID: 24295282

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154585 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor ionotropic, kainate 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 259 amino acids
Theoretical weight: 29.37 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42260 (Residues: 429-544, 667-806; Coverage: 29%)
Gene names: Glur6, Grik2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

4 bound ligands:
Ligand 11W 1 x 11W
Ligand CL 1 x CL
Ligand SO4 1 x SO4
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6122
Unit cell:
a: 102.609Å b: 102.609Å c: 282.513Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.173 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Controlling ionotropic and metabotropic glutamate receptors with light: principles and potential.
Reiner et al. (2015)
2 more

3 mentions without citation

Synapses in the spotlight with synthetic optogenetics.
Berlin et al. (2017)
2 more