PDBe 4gys

X-ray diffraction
2.2Å resolution

Granulibacter bethesdensis allophanate hydrolase co-crystallized with malonate

Released:

Function and Biology Details

Reaction catalysed:
Urea-1-carboxylate + H(2)O = 2 CO(2) + 2 NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Allophanate hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 621 amino acids
Theoretical weight: 66.54 KDa
Source organism: Granulibacter bethesdensis CGDNIH1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0BRB0 (Residues: 1-592; Coverage: 100%)
Gene name: GbCGDNIH1_1744
Sequence domains: Amidase
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P61
Unit cell:
a: 77.701Å b: 77.701Å c: 395.473Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.178 0.203
Expression system: Escherichia coli