› 4gw0

X-ray diffraction
2.45Å resolution

Crystal structure of arginine kinase in complex with imino-L-ornithine, MgADP, and nitrate.

Released:
DOI: 10.2210/pdb4gw0/pdb
PDB entry 4gw0 coloured by chain, front view.
PDB entry 4gw0 coloured by chain, side view.
PDB entry 4gw0 coloured by chain, top view.
Source organism: Limulus polyphemus
Primary publication:
Crystal structures of arginine kinase in complex with ADP, nitrate, and various phosphagen analogs.
Clark SA, Davulcu O, Chapman MS
Biochem Biophys Res Commun 427 212-7 (2012)
PMID: 22995310

Function and Biology Details

Reaction catalysed: 
ATP + L-arginine = ADP + N(omega)-phospho-L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156357 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginine kinase Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 40.25 KDa
Source organism: Limulus polyphemus
Expression system: Escherichia coli
UniProt:
  • Canonical: P51541 (Residues: 1-357; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
Ligand ILO 1 x ILO
Ligand NO3 1 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 65.184Å b: 71.103Å c: 79.962Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.21
Expression system: Escherichia coli

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Citations

1 review citation

Natural Products Containing 'Rare' Organophosphorus Functional Groups.
Petkowski et al. (2019)
6 other articles

1 mention without citation

Crystal structures of arginine kinase in complex with ADP, nitrate, and various phosphagen analogs.
Clark et al. (2012)