PDBe 4gsl

X-ray diffraction
2.7Å resolution

Crystal structure of an Atg7-Atg3 crosslinked complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin-like modifier-activating enzyme ATG7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 615 amino acids
Theoretical weight: 69.69 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38862 (Residues: 1-613; Coverage: 97%)
Gene names: APG7, ATG7, CVT2, YHR171W
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain
Autophagy-related protein 3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 312 amino acids
Theoretical weight: 35.97 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40344 (Residues: 1-310; Coverage: 100%)
Gene names: APG3, ATG3, AUT1, N2040, YNR007C
Sequence domains:
Structure domains: Yope Regulator; Chain: A,

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 281.556Å b: 125.083Å c: 71.079Å
α: 90° β: 103.32° γ: 90°
R-values:
R R work R free
0.194 0.193 0.246
Expression system: Escherichia coli BL21(DE3)