› 4gk8

X-ray diffraction
1.93Å resolution

Crystal structure of histidinol phosphate phosphatase (HISK) from Lactococcus lactis subsp. lactis Il1403 complexed with ZN and L-histidinol arsenate

Released:
DOI: 10.2210/pdb4gk8/pdb
PDB entry 4gk8 coloured by chain, front view.
PDB entry 4gk8 coloured by chain, side view.
PDB entry 4gk8 coloured by chain, top view.
Source organism: Lactococcus lactis subsp. lactis Il1403
Primary publication:
Structural and mechanistic characterization of L-histidinol phosphate phosphatase from the polymerase and histidinol phosphatase family of proteins.
Ghodge SV, Fedorov AA, Fedorov EV, Hillerich B, Seidel R, Almo SC, Raushel FM
Biochemistry 52 1101-12 (2013)
PMID: 23327428

Function and Biology Details

Reaction catalysed: 
L-histidinol phosphate + H(2)O = L-histidinol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histidinol-phosphatase Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 33.24 KDa
Source organism: Lactococcus lactis subsp. lactis Il1403
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02150 (Residues: 2-269; Coverage: 100%)
Gene names: L37351, LL1216, hisK
Sequence domains: PHP domain
Structure domains: Metal-dependent hydrolases

Ligands and Environments

5 bound ligands:
Ligand ZN 4 x ZN
Ligand GK8 1 x GK8
Ligand CL 3 x CL
Ligand IMD 1 x IMD
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 85.86Å b: 86.622Å c: 45.093Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.196
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks.
Gerlt et al. (2015)
11 other articles

1 mention without citation

Structural and mechanistic characterization of L-histidinol phosphate phosphatase from the polymerase and histidinol phosphatase family of proteins.
Ghodge et al. (2013)