› 4gin

X-ray diffraction
1.9Å resolution

Crystal structure of the MUTB R284C mutant from crystals soaked with the inhibitor deoxynojirimycin

Released:
DOI: 10.2210/pdb4gin/pdb
PDB entry 4gin coloured by chain, front view.
PDB entry 4gin coloured by chain, side view.
PDB entry 4gin coloured by chain, top view.
Source organism: Rhizobium sp. MX-45
Primary publication:
Mutations inducing an active-site aperture in Rhizobium sp. sucrose isomerase confer hydrolytic activity.
Lipski A, Watzlawick H, Ravaud S, Robert X, Rhimi M, Haser R, Mattes R, Aghajari N
Acta Crystallogr D Biol Crystallogr 69 298-307 (2013)
PMID: 23385465

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125905 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 584 amino acids
Theoretical weight: 66.82 KDa
Source organism: Rhizobium sp. MX-45
Expression system: Escherichia coli
UniProt:
  • Canonical: M1E1F6 (Residues: 1-584; Coverage: 100%)
Gene name: mutB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 83.8Å b: 89.7Å c: 91.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.14 0.181
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Current studies on sucrose isomerase and biological isomaltulose production using sucrose isomerase.
Mu et al. (2014)
1 more

1 mention without citation

Tumor pyruvate kinase M2 modulators: a comprehensive account of activators and inhibitors as anticancer agents.
Rathod et al. (2021)