› 4g5s

X-ray diffraction
3.62Å resolution

Structure of LGN GL3/Galphai3 complex

Released:
DOI: 10.2210/pdb4g5s/pdb
PDB entry 4g5s coloured by chain, front view.
PDB entry 4g5s coloured by chain, side view.
PDB entry 4g5s coloured by chain, top view.
Source organisms:
Entry authors: Jia M, Li J, Zhu J, Wen W, Zhang M, Wang W

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140371 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 330 amino acids
Theoretical weight: 37.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08754 (Residues: 25-354; Coverage: 93%)
Gene name: GNAI3
Sequence domains: G-protein alpha subunit
Structure domains:
G-protein-signaling modulator 2 Chains: E, F, G, Z
Molecule details ›
Chains: E, F, G, Z
Length: 25 amino acids
Theoretical weight: 2.86 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q8VDU0 (Residues: 594-618; Coverage: 4%)
Gene names: Gpsm2, Lgn, Pins
Sequence domains: GoLoco motif

Ligands and Environments

2 bound ligands:
Ligand GDP 3 x GDP
Ligand CIT 2 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6122
Unit cell:
a: 209.68Å b: 209.68Å c: 235.482Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.207 0.253
Expression systems:
  • Escherichia coli
  • Not provided

Quick links