Function and Biology

Human topoisomerase iibeta in complex with DNA and ametantrone

Source organisms:
Biochemical function: ATP binding
Biological process: DNA topological change
Cellular component: not assigned

EC 5.99.1.3: DNA topoisomerase (ATP-hydrolyzing)

Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Comments:
  • Can introduce negative superhelical turns into double-stranded circular DNA.
  • One unit has nicking-closing activity, and another catalyzes super- twisting and hydrolysis of ATP (cf. EC 5.99.1.2).
Systematic name:
DNA topoisomerase (ATP-hydrolyzing)
Alternative Name(s):
  • DNA topoisomerase II
  • DNA-gyrase
  • Deoxyribonucleate topoisomerase
  • Deoxyribonucleic topoisomerase
  • Topoisomerase
  • Type II DNA topoisomerase
  • DNA gyrase

GO terms

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Sequence families

Pfam Protein families (Pfam)
PF01751
Domain description: Toprim domain
Occurring in:
  1. DNA topoisomerase 2-beta
1 copy of Pfam domain PF01751 (Toprim domain) in DNA topoisomerase 2-beta in PDB 4g0w.

PF00521
Domain description: DNA gyrase/topoisomerase IV, subunit A
Occurring in:
  1. DNA topoisomerase 2-beta
1 copy of Pfam domain PF00521 (DNA gyrase/topoisomerase IV, subunit A) in DNA topoisomerase 2-beta in PDB 4g0w.

PF16898
Domain description: C-terminal associated domain of TOPRIM
Occurring in:
  1. DNA topoisomerase 2-beta
1 copy of Pfam domain PF16898 (C-terminal associated domain of TOPRIM) in DNA topoisomerase 2-beta in PDB 4g0w.

InterPro InterPro annotations
IPR001154
Domain description: DNA topoisomerase II, eukaryotic-type
Occurring in:
  1. DNA topoisomerase 2-beta
IPR002205
Domain description: DNA topoisomerase, type IIA, subunit A/C-terminal
Occurring in:
  1. DNA topoisomerase 2-beta
IPR006171
Domain description: TOPRIM domain
Occurring in:
  1. DNA topoisomerase 2-beta
IPR013759
Domain description: DNA topoisomerase, type IIA, subunit B, C-terminal
Occurring in:
  1. DNA topoisomerase 2-beta
IPR001241
Domain description: DNA topoisomerase, type IIA
Occurring in:
  1. DNA topoisomerase 2-beta
IPR018522
Domain description: DNA topoisomerase, type IIA, conserved site
Occurring in:
  1. DNA topoisomerase 2-beta
IPR024946
Domain description: Arginine repressor-like, C-terminal
Occurring in:
  1. DNA topoisomerase 2-beta
IPR034157
Domain description: DNA topoisomerase 2, TOPRIM domain
Occurring in:
  1. DNA topoisomerase 2-beta
IPR013760
Domain description: DNA topoisomerase, type IIA-like domain superfamily
Occurring in:
  1. DNA topoisomerase 2-beta
IPR031660
Domain description: C-terminal associated domain of TOPRIM
Occurring in:
  1. DNA topoisomerase 2-beta
IPR013757
Domain description: Type IIA DNA topoisomerase subunit A, alpha-helical domain superfamily
Occurring in:
  1. DNA topoisomerase 2-beta
IPR013758
Domain description: DNA topoisomerase, type IIA, subunit A/ C-terminal, alpha-beta
Occurring in:
  1. DNA topoisomerase 2-beta

Structure domains

CATH CATH domains
3.90.199.10
Class: Alpha Beta
Architecture: Alpha-Beta Complex
Topology: Topoisomerase II; domain 5
Homology: Topoisomerase II, domain 5
Occurring in:
  1. DNA topoisomerase 2-beta
1 copy of CATH domain 3.90.199.10 (Topoisomerase II; domain 5) in DNA topoisomerase 2-beta in PDB 4g0w.
3.40.50.670
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: Rossmann fold
Occurring in:
  1. DNA topoisomerase 2-beta
1 copy of CATH domain 3.40.50.670 (Rossmann fold) in DNA topoisomerase 2-beta in PDB 4g0w.
3.30.1360.40
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Gyrase A; domain 2
Homology: Gyrase A; domain 2
Occurring in:
  1. DNA topoisomerase 2-beta
1 copy of CATH domain 3.30.1360.40 (Gyrase A; domain 2) in DNA topoisomerase 2-beta in PDB 4g0w.
1.10.268.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Topoisomerase; domain 3
Homology: Topoisomerase, domain 3
Occurring in:
  1. DNA topoisomerase 2-beta
1 copy of CATH domain 1.10.268.10 (Topoisomerase; domain 3) in DNA topoisomerase 2-beta in PDB 4g0w.