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Crystal structure of MST4-MO25 complex with WSF motif

X-ray diffraction
3.25Å resolution
PDB entry 4fzd coloured by chain, front view.
PDB entry 4fzd coloured by chain, side view.
PDB entry 4fzd coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Serine/threonine-protein kinase 26 and Calcium-binding protein 39
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Multimeric state: hetero trimer
Accessible surface area: 29826.05 Å2
Buried surface area: 2386.69 Å2
Dissociation area: 1,047.7 Å2
Dissociation energy (ΔGdiss): -3.45 kcal/mol
Dissociation entropy (TΔSdiss): 19.21 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-192830

Macromolecules

Calcium-binding protein 39
Chain: A
Length: 328 amino acids
Theoretical weight: 38.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y376 (Residues: 11-334; Coverage: 95%)
Gene names: CAB39, CGI-66, MO25
Pfam: Mo25-like
InterPro:
CATH: Leucine-rich Repeat Variant
Calcium-binding protein 39 in PDB entry 4fzd, assembly 1, front view.
Calcium-binding protein 39 in PDB entry 4fzd, assembly 1, side view.
Calcium-binding protein 39 in PDB entry 4fzd, assembly 1, top view.
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Serine/threonine-protein kinase 26
Chain: B
Length: 283 amino acids
Theoretical weight: 31.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9P289 (Residues: 18-297; Coverage: 67%)
Gene names: MASK, MST4, STK26
Pfam: Protein kinase domain
InterPro:
CATH:
Serine/threonine-protein kinase 26 in PDB entry 4fzd, assembly 1, front view.
Serine/threonine-protein kinase 26 in PDB entry 4fzd, assembly 1, side view.
Serine/threonine-protein kinase 26 in PDB entry 4fzd, assembly 1, top view.
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Serine/threonine-protein kinase 26
Chain: C
Length: 5 amino acids
Theoretical weight: 669 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9P289 (Residues: 323-327; Coverage: 1%)
Gene names: MASK, MST4, STK26
Serine/threonine-protein kinase 26 in PDB entry 4fzd, assembly 1, front view.
Serine/threonine-protein kinase 26 in PDB entry 4fzd, assembly 1, side view.
Serine/threonine-protein kinase 26 in PDB entry 4fzd, assembly 1, top view.
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