PDBe 4fxd

X-ray diffraction
3Å resolution

Crystal structure of yeast DNA polymerase alpha bound to DNA/RNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
DNA polymerase alpha catalytic subunit A Chains: A, B
Molecule details ›
Chains: A, B
Length: 910 amino acids
Theoretical weight: 103.53 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13382 (Residues: 349-1258; Coverage: 62%)
Gene names: CDC17, N2181, POL1, YNL102W
Sequence domains:
Structure domains:
DNA (5'-D(*TP*TP*TP*TP*CP*GP*CP*TP*GP*CP*CP*CP*GP*CP*CP*T)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 nucleotides
Theoretical weight: 4.79 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA (5'-R(*AP*GP*GP*CP*GP*GP*GP*CP*AP*G)-3') Chains: E, F
Molecule details ›
Chains: E, F
Length: 10 nucleotides
Theoretical weight: 3.3 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P1
Unit cell:
a: 72.14Å b: 74.77Å c: 116.99Å
α: 82.26° β: 72.57° γ: 82.4°
R-values:
R R work R free
0.256 0.254 0.285
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided