› 4frf

X-ray diffraction
2.9Å resolution

Structural Studies and Protein Engineering of Inositol Phosphate Multikinase

Released:
DOI: 10.2210/pdb4frf/pdb
PDB entry 4frf coloured by chain, front view.
PDB entry 4frf coloured by chain, side view.
PDB entry 4frf coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural studies and protein engineering of inositol phosphate multikinase.
Endo-Streeter S, Tsui MM, Odom AR, Block J, York JD
J Biol Chem 287 35360-35369 (2012)
PMID: 22896696

Function and Biology Details

Reactions catalysed: 
ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate = ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
(1a) ATP + 1D-myo-inositol 1,4,5-trisphosphate = ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol polyphosphate multikinase alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 275 amino acids
Theoretical weight: 30.76 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9LY23 (Residues: 16-286; Coverage: 95%)
Gene names: At5g07370, IP3K, IPK2a, IPMK, T2I1.80
Sequence domains: Inositol polyphosphate kinase
Structure domains: Inositol polyphosphate kinase

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P3221
Unit cell:
a: 130.79Å b: 130.79Å c: 129.93Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.239 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Inositol phosphate kinases: Expanding the biological significance of the universal core of the protein kinase fold.
Shears et al. (2019)
4 more

4 mentions without citation

The enzymes of human diphosphoinositol polyphosphate metabolism.
Thomas et al. (2014)
3 more