› 4fgy

X-ray diffraction
2.84Å resolution

Identification of a unique PPAR ligand with an unexpected binding mode and antibetic activity

Released:
DOI: 10.2210/pdb4fgy/pdb
PDB entry 4fgy coloured by chain, front view.
PDB entry 4fgy coloured by chain, side view.
PDB entry 4fgy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification of the antibiotic ionomycin as an unexpected peroxisome proliferator-activated receptor γ (PPARγ) ligand with a unique binding mode and effective glucose-lowering activity in a mouse model of diabetes.
Zheng W, Feng X, Qiu L, Pan Z, Wang R, Lin S, Hou D, Jin L, Li Y
Diabetologia 56 401-11 (2013)
PMID: 23178929

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153375 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peroxisome proliferator-activated receptor gamma Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 30.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37231 (Residues: 235-504; Coverage: 54%)
Gene names: NR1C3, PPARG
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 1 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.45 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15788 (Residues: 686-696; Coverage: 1%)
Gene names: BHLHE74, NCOA1, SRC1

Ligands and Environments

1 bound ligand:
Ligand 0W3 1 x 0W3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2221
Unit cell:
a: 57.36Å b: 86.527Å c: 122.691Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.285
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

11 review citations

Ligands for the Nuclear Peroxisome Proliferator-Activated Receptor Gamma.
Sauer S. (2015)
10 more

3 mentions without citation

Cooperative cobinding of synthetic and natural ligands to the nuclear receptor PPARγ.
Shang et al. (2018)
2 more