› 4fd8

X-ray diffraction
1.52Å resolution

Structure of apo S70C SHV beta-lactamase

Released:
DOI: 10.2210/pdb4fd8/pdb
PDB entry 4fd8 coloured by chain, front view.
PDB entry 4fd8 coloured by chain, side view.
PDB entry 4fd8 coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Crystal structure of a preacylation complex of the β-lactamase inhibitor sulbactam bound to a sulfenamide bond-containing thiol-β-lactamase.
Rodkey EA, Drawz SM, Sampson JM, Bethel CR, Bonomo RA, van den Akker F
J Am Chem Soc 134 16798-804 (2012)
PMID: 22974281

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142332 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase SHV-1 Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 28.92 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AD64 (Residues: 22-286; Coverage: 100%)
Gene names: bla, blaSHV-1, shv1
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

1 bound ligand:
Ligand MA4 2 x MA4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 49.354Å b: 55.584Å c: 84.414Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.197
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Redox Signaling by Reactive Electrophiles and Oxidants.
Parvez et al. (2018)
1 more

2 mentions without citation

Crystal structure of a preacylation complex of the β-lactamase inhibitor sulbactam bound to a sulfenamide bond-containing thiol-β-lactamase.
Rodkey et al. (2012)
1 more