PDBe 4fbu

X-ray diffraction
2.6Å resolution

Dpo4 polymerase pre-insertion binary complex with the N-(deoxyguanosin-8-yl)-1-aminopyrene lesion

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase IV Chains: A, B
Molecule details ›
Chains: A, B
Length: 341 amino acids
Theoretical weight: 39.02 KDa
Source organism: Sulfolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97W02 (Residues: 1-341; Coverage: 97%)
Gene names: SSO2448, dbh, dpo4
Sequence domains:
Structure domains:
DNA primer Chains: D, P
Molecule details ›
Chains: D, P
Length: 13 nucleotides
Theoretical weight: 3.88 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA template Chains: C, T
Molecule details ›
Chains: C, T
Length: 14 nucleotides
Theoretical weight: 4.61 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 52.774Å b: 181.458Å c: 52.744Å
α: 90° β: 109.49° γ: 90°
R-values:
R R work R free
0.22 0.218 0.261
Expression systems:
  • Escherichia coli
  • Not provided