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Structure of the Cif:Nedd8 complex - Photorhabdus luminescens Cycle Inhibiting Factor in complex with human Nedd8

X-ray diffraction
1.6Å resolution
PDB entry 4fbj coloured by chain, front view.
PDB entry 4fbj coloured by chain, side view.
PDB entry 4fbj coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: NEDD8 and Protein-glutamine deamidase Cif
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Multimeric state: hetero dimer
Accessible surface area: 14639.82 Å2
Buried surface area: 2810.32 Å2
Dissociation area: 1,336.41 Å2
Dissociation energy (ΔGdiss): 6.44 kcal/mol
Dissociation entropy (TΔSdiss): 11.18 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-172427

Macromolecules

Protein-glutamine deamidase Cif
Chain: A
Length: 261 amino acids
Theoretical weight: 29.64 KDa
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7N439 (Residues: 53-313; Coverage: 83%)
Gene names: cif, plu2515
Pfam: Cycle inhibiting factor (CIF)
InterPro: Protein-glutamine deamidase Cif
Protein-glutamine deamidase Cif in PDB entry 4fbj, assembly 1, front view.
Protein-glutamine deamidase Cif in PDB entry 4fbj, assembly 1, side view.
Protein-glutamine deamidase Cif in PDB entry 4fbj, assembly 1, top view.
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NEDD8
Chain: B
Length: 88 amino acids
Theoretical weight: 10.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15843 (Residues: 1-81; Coverage: 100%)
Gene name: NEDD8
Pfam: Ubiquitin family
InterPro:
CATH: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
NEDD8 in PDB entry 4fbj, assembly 1, front view.
NEDD8 in PDB entry 4fbj, assembly 1, side view.
NEDD8 in PDB entry 4fbj, assembly 1, top view.
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