PDBe 4fbj

X-ray diffraction
1.6Å resolution

Structure of the Cif:Nedd8 complex - Photorhabdus luminescens Cycle Inhibiting Factor in complex with human Nedd8

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NEDD8 Chain: B
Molecule details ›
Chain: B
Length: 88 amino acids
Theoretical weight: 10.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15843 (Residues: 1-81; Coverage: 100%)
Gene name: NEDD8
Sequence domains: Ubiquitin family
Hypothetical protein Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 29.64 KDa
Source organism: Photorhabdus luminescens subsp. laumondii TTO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7N439 (Residues: 53-313; Coverage: 83%)
Gene name: plu2515
Sequence domains: Cycle inhibiting factor (CIF)

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 40.73Å b: 56.09Å c: 67.55Å
α: 90° β: 104.13° γ: 90°
R-values:
R R work R free
0.168 0.165 0.236
Expression system: Escherichia coli