PDBe 4f8c

X-ray diffraction
1.95Å resolution

Structure of the Cif:Nedd8 complex - Yersinia pseudotuberculosis Cycle Inhibiting Factor in complex with human Nedd8

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NEDD8 Chains: B, D
Molecule details ›
Chains: B, D
Length: 88 amino acids
Theoretical weight: 10.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15843 (Residues: 1-81; Coverage: 100%)
Gene name: NEDD8
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Cycle Inhibiting Factor Chains: A, C
Molecule details ›
Chains: A, C
Length: 275 amino acids
Theoretical weight: 30.81 KDa
Source organism: Yersinia pseudotuberculosis YPIII
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3B1Q8 (Residues: 33-288; Coverage: 88%)
Gene name: YPK_1971
Sequence domains: Cycle inhibiting factor (CIF)

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P6322
Unit cell:
a: 125.359Å b: 125.359Å c: 169.93Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.187 0.235
Expression system: Escherichia coli