PDBe 4ew6

X-ray diffraction
2.3Å resolution

Crystal structure of D-galactose-1-dehydrogenase protein from Rhizobium etli

Released:
Source organism: Rhizobium etli CFN 42
Entry authors: Eswaramoorthy S, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed:
D-galactose + NAD(+) = D-galactono-1,4-lactone + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-galactose-1-dehydrogenase protein Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 36.6 KDa
Source organism: Rhizobium etli CFN 42
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q2KA75 (Residues: 1-308; Coverage: 100%)
Gene names: RHE_CH01458, gal
Sequence domains: Oxidoreductase family, NAD-binding Rossmann fold
Structure domains:

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P43212
Unit cell:
a: 52.109Å b: 52.109Å c: 265.344Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.244 0.293
Expression system: Escherichia coli BL21