PDBe 4esj

X-ray diffraction
2.05Å resolution

RESTRICTION ENDONUCLEASE DpnI IN COMPLEX WITH TARGET DNA

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates. 
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type-2 restriction enzyme DpnI Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 30.08 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A460 (Residues: 1-254; Coverage: 100%)
Gene names: dpnC, spr1665
Sequence domains: Dam-replacing family
DNA (5'-D(*CP*TP*GP*GP*(6MA)P*TP*CP*CP*AP*G)-3') Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 10 nucleotides
Theoretical weight: 3.06 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 130.518Å b: 98.411Å c: 83.884Å
α: 90° β: 112.21° γ: 90°
R-values:
R R work R free
0.199 0.198 0.218
Expression systems:
  • Escherichia coli
  • Not provided