Function and Biology

Snapshot of the large fragment of DNA polymerase I from Thermus Aquaticus processing modified pyrimidines

Source organisms:
Biochemical function: DNA binding
Biological process: DNA repair
Cellular component: not assigned

EC 2.7.7.7: DNA-directed DNA polymerase

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Comments:
  • Catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time.
  • Cannot initiate a chain de novo.
  • Requires a primer which may be DNA or RNA.
  • See also EC 2.7.7.49.
Systematic name:
Deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (DNA-directed)
Alternative Name(s):
  • DNA duplicase
  • DNA nucleotidyltransferase
  • DNA nucleotidyltransferase (DNA-directed)
  • DNA polymerase
  • DNA polymerase alpha
  • DNA polymerase beta
  • DNA polymerase gamma
  • DNA polymerase I
  • DNA polymerase II
  • DNA polymerase III
  • DNA replicase
  • DNA-dependent DNA polymerase
  • Klenow fragment
  • Taq DNA polymerase
  • Taq Pol I
  • Tca DNA polymerase
  • Deoxynucleate polymerase
  • Deoxyribonucleate nucleotidyltransferase
  • Deoxyribonucleic acid duplicase
  • Deoxyribonucleic acid polymerase
  • Deoxyribonucleic duplicase
  • Deoxyribonucleic polymerase
  • Deoxyribonucleic polymerase I
  • Duplicase
  • Sequenase

GO terms

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Sequence families

Pfam Protein families (Pfam)
PF00476
Domain description: DNA polymerase family A
Occurring in:
  1. DNA polymerase I, thermostable
1 copy of Pfam domain PF00476 (DNA polymerase family A) in DNA polymerase I, thermostable in PDB 4elv.

PF09281
Domain description: Taq polymerase, exonuclease
Occurring in:
  1. DNA polymerase I, thermostable
1 copy of Pfam domain PF09281 (Taq polymerase, exonuclease) in DNA polymerase I, thermostable in PDB 4elv.

InterPro InterPro annotations
IPR002298
Domain description: DNA polymerase A
Occurring in:
  1. DNA polymerase I, thermostable
IPR001098
Domain description: DNA-directed DNA polymerase, family A, palm domain
Occurring in:
  1. DNA polymerase I, thermostable
IPR012337
Domain description: Ribonuclease H-like superfamily
Occurring in:
  1. DNA polymerase I, thermostable
IPR019760
Domain description: DNA-directed DNA polymerase, family A, conserved site
Occurring in:
  1. DNA polymerase I, thermostable
IPR018320
Domain description: DNA polymerase 1
Occurring in:
  1. DNA polymerase I, thermostable
IPR015361
Domain description: Taq polymerase, thermostable, exonuclease region
Occurring in:
  1. DNA polymerase I, thermostable
IPR036397
Domain description: Ribonuclease H superfamily
Occurring in:
  1. DNA polymerase I, thermostable

Structure domains

CATH CATH domains
3.30.70.370
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Alpha-Beta Plaits
Homology: Alpha-Beta Plaits
Occurring in:
  1. DNA polymerase I, thermostable
1 copy of CATH domain 3.30.70.370 (Alpha-Beta Plaits) in DNA polymerase I, thermostable in PDB 4elv.
1.10.150.20
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: DNA polymerase; domain 1
Homology: 5' to 3' exonuclease, C-terminal subdomain
Occurring in:
  1. DNA polymerase I, thermostable
1 copy of CATH domain 1.10.150.20 (DNA polymerase; domain 1) in DNA polymerase I, thermostable in PDB 4elv.
3.30.420.10
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Nucleotidyltransferase; domain 5
Homology: Nucleotidyltransferase; domain 5
Occurring in:
  1. DNA polymerase I, thermostable
1 copy of CATH domain 3.30.420.10 (Nucleotidyltransferase; domain 5) in DNA polymerase I, thermostable in PDB 4elv.
1.20.1060.10
Class: Mainly Alpha
Architecture: Up-down Bundle
Topology: Taq DNA Polymerase; Chain T, domain 4
Homology: Taq DNA Polymerase; Chain T, domain 4
Occurring in:
  1. DNA polymerase I, thermostable
1 copy of CATH domain 1.20.1060.10 (Taq DNA Polymerase; Chain T, domain 4) in DNA polymerase I, thermostable in PDB 4elv.