› 4ej1

X-ray diffraction
1.75Å resolution

Binding of Nb113 camelid antibody fragment with the binary DHFR:folate complex

Released:
DOI: 10.2210/pdb4ej1/pdb
PDB entry 4ej1 coloured by chain, front view.
PDB entry 4ej1 coloured by chain, side view.
PDB entry 4ej1 coloured by chain, top view.
Source organisms:
Primary publication:
Mechanistic analysis of allosteric and non-allosteric effects arising from nanobody binding to two epitopes of the dihydrofolate reductase of Escherichia coli.
Oyen D, Wechselberger R, Srinivasan V, Steyaert J, Barlow JN
Biochim Biophys Acta 1834 2147-57 (2013)
PMID: 23911607

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209581 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dihydrofolate reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 18.02 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABQ4 (Residues: 1-159; Coverage: 100%)
Gene names: JW0047, b0048, folA, tmrA
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A
Nb113 camelid antibody fragment Chains: C, D
Molecule details ›
Chains: C, D
Length: 134 amino acids
Theoretical weight: 14.82 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand FOL 2 x FOL
Ligand PO4 6 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 100.741Å b: 77.064Å c: 92.777Å
α: 90° β: 92° γ: 90°
R-values:
R R work R free
0.188 0.186 0.224
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Camelid nanobodies: killing two birds with one stone.
Desmyter et al. (2015)
3 more

2 mentions without citation

Positive-unlabeled learning for the prediction of conformational B-cell epitopes.
Ren et al. (2015)
1 more