› 4e4h

X-ray diffraction
2.28Å resolution

Crystal structure of Histone Demethylase NO66

Released:
DOI: 10.2210/pdb4e4h/pdb
PDB entry 4e4h coloured by chain, front view.
PDB entry 4e4h coloured by chain, side view.
PDB entry 4e4h coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Wu M, Tao Y, Zang J

Function and Biology Details

Reaction catalysed: 
(1a) a [histone H3]-N(6),N(6)-dimethyl-L-lysine(36) + 2-oxoglutarate + O(2) = a [histone H3]-N(6)-methyl-L-lysine(36) + succinate + formaldehyde + CO(2)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190649 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal oxygenase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 463 amino acids
Theoretical weight: 52.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H6W3 (Residues: 183-641; Coverage: 72%)
Gene names: C14orf169, MAPJD, NO66, RIOX1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand FE 4 x FE
Ligand ACT 1 x ACT
Ligand GOL 1 x GOL
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P32
Unit cell:
a: 89.352Å b: 89.352Å c: 304.863Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.202 0.252
Expression system: Escherichia coli

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