PDBe 4e41

X-ray diffraction
2.6Å resolution

Structural basis for the recognition of mutant self by a tumor-specific, MHC class II-restricted T cell receptor G4

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Triosephosphate isomerase Chains: C, H
Molecule details ›
Chains: C, H
Length: 15 amino acids
Theoretical weight: 1.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60174 (Residues: 60-74; Coverage: 5%)
Gene names: TPI, TPI1
T cell receptor G4 beta chain Chains: E, J
Molecule details ›
Chains: E, J
Length: 239 amino acids
Theoretical weight: 27.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
T cell receptor G4 alpha chain Chains: D, I
Molecule details ›
Chains: D, I
Length: 203 amino acids
Theoretical weight: 22.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
HLA class II histocompatibility antigen, DR alpha chain Chains: A, F
Molecule details ›
Chains: A, F
Length: 182 amino acids
Theoretical weight: 21.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01903 (Residues: 26-207; Coverage: 80%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1-1 beta chain Chains: B, G
Molecule details ›
Chains: B, G
Length: 190 amino acids
Theoretical weight: 22.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04229 (Residues: 30-219; Coverage: 80%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 126.133Å b: 175.611Å c: 88.646Å
α: 90° β: 110.75° γ: 90°
R-values:
R R work R free
0.201 0.197 0.264
Expression system: Escherichia coli