› 4dyy

X-ray diffraction
1.9Å resolution

Crystal Structure of the Cu-adduct of Human H-Ferritin variant MIC1

Released:
DOI: 10.2210/pdb4dyy/pdb
PDB entry 4dyy coloured by chain, front view.
PDB entry 4dyy coloured by chain, side view.
PDB entry 4dyy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Re-engineering protein interfaces yields copper-inducible ferritin cage assembly.
Huard DJ, Kane KM, Tezcan FA
Nat Chem Biol 9 169-76 (2013)
PMID: 23340339

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 172 amino acids
Theoretical weight: 20.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02794 (Residues: 6-177; Coverage: 94%)
Gene names: FTH, FTH1, FTHL6, OK/SW-cl.84, PIG15
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

4 bound ligands:
Ligand CU 3 x CU
Ligand CA 2 x CA
Ligand EDO 1 x EDO
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: F432
Unit cell:
a: 179.594Å b: 179.594Å c: 179.594Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.218 0.241
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
16 more

1 mention without citation

Strategies to control the binding mode of de novo designed protein interactions.
Der et al. (2013)