› 4dkd

X-ray diffraction
3Å resolution

Crystal Structure of Human Interleukin-34 Bound to Human CSF-1R

Released:
DOI: 10.2210/pdb4dkd/pdb
PDB entry 4dkd coloured by chain, front view.
PDB entry 4dkd coloured by chain, side view.
PDB entry 4dkd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R.
Ma X, Lin WY, Chen Y, Stawicki S, Mukhyala K, Wu Y, Martin F, Bazan JF, Starovasnik MA
Structure 20 676-87 (2012)
PMID: 22483114

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139515 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-34 Chains: A, B
Molecule details ›
Chains: A, B
Length: 185 amino acids
Theoretical weight: 21.43 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6ZMJ4 (Residues: 21-193; Coverage: 78%)
Gene names: C16orf77, IL34
Sequence domains: Interleukin 34
Structure domains: Interleukin-34
Macrophage colony-stimulating factor 1 receptor Chain: C
Molecule details ›
Chain: C
Length: 292 amino acids
Theoretical weight: 32.04 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P07333 (Residues: 20-299; Coverage: 29%)
Gene names: CSF1R, FMS
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand BMA 2 x BMA
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3121
Unit cell:
a: 101.361Å b: 101.361Å c: 175.252Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.254 0.252 0.287
Expression system: Trichoplusia ni

Quick links

Citations

39 review citations

From Monocytes to M1/M2 Macrophages: Phenotypical vs. Functional Differentiation.
Italiani et al. (2014)
38 more

5 mentions without citation

Cloning and expression of porcine Colony Stimulating Factor-1 (CSF-1) and Colony Stimulating Factor-1 Receptor (CSF-1R) and analysis of the species specificity of stimulation by CSF-1 and Interleukin 34.
Gow et al. (2012)
4 more